PRODUCTION AND CHARACTERIZATION OF ELECTROSPUN NANOFIBERS, SUITABLE FOR USE IN TISSUE ENGINEERING
Project no. 22175 · Status: Kapanmış · 2020
Summary
<p>In this study, electrospun nanofibers were developed for use in the field of skin tissue </p><p>engineering as scaffold and wound dressing material. Nanofibers were prepared with poly(ε-</p><p>caprolactone) (PCL), Car and NSO for the purpose of developing the skin tissue engineering matrices for wound healing. Cell viability experiments were performed on HaCaT cell line for the choosing of appropriate PCL-Car/NSO nanofibers via fluorescence microscopy with DAPI, and wound healing activity was investigated with culture inserts performed. 10 mg/mL NSO containing PCL-Car/NSO nanofibers were found </p><p>more effective at wound healing compared with the control 43.16 ± 15.56% and 48.33 ± 14.72% </p><p>for 48 and 72 hours, respectively. PCL, CA and MA were used for the development of nanofibrous bioactive wound dressing material. PCL-CA-MA nanofibers’ effects on viability of HaCaT cell lines were determined by MTT. PCL-CA nanofibers, which contained 25 mM MA, were found the most effective nanofiber formulation. </p><p>PCL-CA-MA (25 mM) nanofibers showed an excellent wound healing activity (39.35±12.46%; 65.31±10.74% and 94.78±6.77% for 4, 6 and 8 hours, respectively) compared with control group, (17.88±3.34%; 19.36±6.64% and 29.07±7.66% for 4, 6 and 8 hours, respectively) in a very short time. </p>