CLONING OF THE XYLANASE GENE FROM THERMOPHILIC ACTINOMISET Thermobifida fusca EFTL 17 STRAINS AND PRODUCTION OF RECOMBINANT PROTEIN
Project no. 573 · Status: Kapanmış · 2014
Summary
In this thesis, the isolation of the xylanase gene from the Thermobifida fusca EFTL 17 strain and the cloning of the xylanase gene using eukaryotic and prokaryotic hosts and the production of recombinant protein have been investigated. In the thesis, izaolates obtained from the compost of the old Foça mushroom farm were developed in a modified xylan (+) TA medium containing xylan, and the enzyme activity under different conditions of xylanase was checked. It has been determined that the enzyme exhibits optimum activity in xylan (+) TA medium, at 55 ° C, pH 7,2, 166,5 U/mL. At this stage of the thesis, the gene region transducing the xylanase of Thermobifida fusca EFTL 17 was cloned into E. coli and the transformation amount and colony number were found to be lower than expected.