Umbilical Cord Mesenchymal Stem Cells (UC-MSC) Culture Investigation of Platelet-Rich Plasma (PRP) Efficiency
Project no. 24008 · Status: Kapanmış (Yayın bekleniyor) · 2022
Summary
<p>Mesenchymal stem cells (MSCs) are multipotent stem cells that can be obtained from many tissues; mainly from adipose tissue, cord blood, umbilical cord, etc. MSCs have recently attracted much attention in regenerative stem cell applications; due to their paracrine effects following tissue damage and their immunosuppressive/immunomodulatory properties. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are MSCs that can be easily isolated, used allogeneically, and have strong immunomodulatory/anti-inflammatory effects.</p><p>Fetal bovine serum (FBS) is frequently used in special solutions that meet the nutritional needs of the cells in cell culture. However, since commercially purchased solutions are high in cost or contain animal products, alternative solutions are also sought. Platelet-rich plasma (PRP), obtained from human blood, can replace FBS since it is an autologous product containing growth hormones and plasma proteins necessary for the development of cells. For this reason, in our study, PRP was obtained from peripheral blood samples taken from volunteers using a two-stage centrifuge technique. Instead of FBS, human serum or PRP with/without activator was applied to the culture medium that allows UC-MSC to be obtained, and their effects on the growth and proliferation of cells in culture were comparatively examined. In the culture of UC-MSC, it was observed that approximately 2 times more live cells were obtained in PRP culture medium with CaCl2 activation for 15 minutes/overnight compared to the culture medium using only FBS /PRP/human serum (p < 0.0001).</p><p> As a result, it was determined that using PRP with CaCl2 activation instead of FBS was the most effective culture method in the culture of UC-MSC. In our study, a culturing technique that provides effective UC-MSCs suitable for allogeneic use was developed and its superiority compared to other commonly used methods was determined. Since this UC-MSC culture technique is very suitable for widespread use, it can be easily recommended for transferring it to experimental and clinical studies in the future.</p>