Evaluation Of The Efficiency Of Epigallocatechin Gallate, Resveratrol And Autologous Serum In The Model Of Chemical Eye Injury
Project no. 20952 · Status: Kapanmış · 2020
Summary
<p>Chemical eye injuries are still one of the important ophthalmological emergencies with complications that are difficult to cope with. Many biomolecules are currently being investigated to promote corneal epithelial healing after chemical injury. The aim of this study is to evaluate the effectiveness of epigallocatechin gallic acid and resveratrol, which have antioxidant and anti-inflammatory properties, in accelerating epithelial healing after chemical burns. 15 rabbits belongs to New Zealand genus were used. The rabbits were divided to 3 groups. Each group contained 5 rabbits. 35-50 mg/kg ketamine and 5-10 mg/kg xsilazine were given to all rabbits subcutaneously. The membrane filtered papers impregnated with %10 potassium hydroxide were placed over the right eyes of the rabbits and waited for 30 seconds to obtain a chemical burn. The ocular surfaces were washed over with %0,9 saline for 10 minutes. In group 1, epigallocatechin gallate solution was dropped to right eye; in group 2, resveratrol solution was dropped to right eye and in group 3, %20 autologous serum was dropped to right eye for 3 weeks. In all groups, %0,9 saline was dropped to left eye for 3 weeks. In all groups, the eyes were photographed for 3 weeks during the experiment. Afterwards all of the rabbits were sacrificed and their corneas were excised. The corneas were examined histopathologically. Hematoxylin Eosin, Masson Trichrom and PAS stainings were applied in histopathological examination. In addition, examination was performed with GFAP, Ki-67 and NF kB and IL-17 immunohistochemically. Two more rabbits were used for the control group, regardless of the research budget. Chemical burn was induced in the right eye of the rabbits in the control group with the same method. %0,9 saline was dropped for 3 weeks and the corneas of control groups were excised and examined histopathologically.</p>