Investigation of the Anti-Cancer Efficiency of Natural Killer Cells Differentiated from Adipose Tissue-Derived Mesenchymal Stem Cells Co-cultured with Pancreatic Cancer Stem Cells
Project no. 13 · Status: Kapanmış · 2016
Summary
Pancreatic cancer (PaCa) is one of the most lethal human cancer types with a 5-year survival rate of 3-5% and a life span of approximately 6 months. MSCs are cells suitable for in vitro propagation. Generally, adipose tissue, which can be obtained easily and abundantly by non-invasive surgical procedures, is a rich source of MSCs. Cancer stem cells can regenerate themselves, that are capable of forming colonies in other tissues and they are resistant in chemical stimuli and radiation. Natural killer (NK) cells play an important role in host immunity in case of malignancy and infection. Several studies have shown that NK cells mediate strong antitumor responses. In our study; commercially available AD-MSCs were cultured in recommended culture media untill it reached to confluency. Cultured AD-MSCs gained hematopoietic characteristics after hematopoietic induction and then were differantiated to NK cells. NK cells were characterised by flowcytometri and immunohistochemical analysis via labeling the cells with CD314/NKG2D antibody. The apoptotic activity of NK cells on PaCa cancer stem cells was determined by annexin V and TUNEL assays.