The effect of chronic anaerobic training (CANT) on basic hematological parameters and the role of erythropoietin (EPO C/A) polymorphism.

Project no. 22889 · Status: Kapanmış · 2021

Summary

Objective: Frequent repetitive hypoxic movements during anaerobic training may affect hypoxia-induced factor (HIF) and EPO levels, as well as some basic haematological parameters, and may have a role in a polymorphism (EPOP) such as EPO rs1617640 (EPO C/A) observed in EPO. However, neither aerobic nor anaerobic training has basic hematological parameters [serum iron, ferritin, transferrin and total iron binding capacity (TIBC)], as well as serum EPO, hypoxia-induced factor 1 and 2 α (HIF1α, HIF-2α) and oxidative stress. The role of the frequently observed EPOP in its possible effect on (OS) indicators is unknown. Therefore, the aim of this study is; The effect of CANT on the above-mentioned parameters and the investigation of the role of EPOP in these possible effects.Methods and Methods: Athlete group (SG, n=35) and control group (KG, n=38) participated in the YOYO intermittent recovery (YOYO IR-1) test. Basic hematological parameters mentioned above and serum EPO, HIF-1α, HIF-2α and interleukin 6 (IL-6) levels (by ELISA method), as well as serum C-reactive protein (CRP), alanine and aspartate aminotransferase (ALT and AST) enzyme activities and oxidative stress index (OSI = oxidant/antioxidant status ratio (TOS/TAS ratio) were determined from fed blood. EPOP was determined from DNA samples isolated from peripheral blood.Results: When polymorphism is not taken into account; The HIF2α value of SG was significantly greater than that of KG (p=0.049), whereas its RBC (p=0.033), HCT (p=0.018) and HGB (%25, p>0.05) values were lower than those of KG. There was no significant difference between the groups in terms of HIF2α, EPO, TOS and OSI levels (p>0.05). The MCV value of the athlete CA heterozygous group (p=0.044) was significantly higher than the CC homozygous group. Considering the polymorphism; In the athlete genotype groups, the RBC, HCT, ferritin (20%) and TAS values of the CC group were significantly lower than those of the control group, whereas the HIF-2α, TOS and OSI (50%) values of the same group were higher than those of the control group. Although the serum EPO values of the athlete AA homozygous group were not significant, they were approximately 28.4% greater than those of both the athlete and control genotype groups.Conclusions: These findings indicate that chronic anaerobic training decreases RBC, HGB, and HCT values, while increasing serum HIF2α values. However, taking into account the polymorphism: A) Although the ferritin concentrations of the CC homozygous group tended to be genetically higher than both the AA homozygous and CA heterozygous groups, its hematological parameters were adversely affected by the training; B) The fact that this group had higher OS levels compared to the others may have played a role in the adverse effects of anaerobic training on hematological parameters of the CC group; C) It can be said that the AA homozygous group has a higher EPO level than the other genotype groups and tends to benefit more from anaerobic training. These findings are reorted first in the literature.

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