Investigation of the Immunomodulator Effectiveness of Beta–Glucan and Cephalaria Saponin Combinations
Project no. 22576 · Status: Kapanmış (Yayın bekleniyor) · 2021
Summary
The immune system includes all cells and molecules that play a role in the defense mechanism against inflammatory damage. Macrophages with both innate and adaptive immune responses are polarized to macrophages with the anti-inflammatory M2 or pro-inflammatory M1 phenotype, with the entry of monocytes into the tissue. Innate immune modulators are key to controlling and repairing damage that affects the immune system. Studies have reported that saponins induce the production of cytokines such as interleukins and interferons that may mediate their immunostimulating effects. ß-glucans are known to activate macrophages, dendritic, neutrophils, B cells, T cells and natural killer cells. In this thesis, it was aimed to investigate the immunomodulatory activity of Scoposide A, Scoposide B, Aristatoside B, Elmalienoside A, Balanoide B saponin molecules isolated from Cephalaria spp., ß-glucan and ß-glucan saponin combinations on THP-1 macrophage cell. In the thesis study, THP-1 monocyte cells were differentiated to THP-1 macrophage with PMA by 48 hours incubation.Then, Scoposide A, Scoposide B, Aristatoside B, Elmalienoside A, Balanoide B, ß-glucan and ß-glucan saponin combinations are added on macrophage. After 24 hours incubation, by staining macrophage cells with FITC-CD11b, FITC-CD163 and PE-CD80 antibodies and effect of macrophage polarization in flow cytometer has been determined. When we look at the results of the study, it was observed that the combination of ß-glucan and saponin ß-glucan both reduced pro-inflammation and promoted pro-inflammatory in THP-1 cells of macrophage origin. This gave results supporting the Th1/ Th2 response. Combinations of ß-glucan and saponin may be potential for cancer studies, reduction of tumor size, immunotherapeutic agents by increasing the level of basic immune response, for individuals with impaired immune response.