IN VITRO REGENERATION OF VIGNA CARACALLA L. VERDC. IN LIQUID MEDIA
Project no. 21682 · Status: Kapanmış · 2020
Summary
In this thesis, Vigna caracalla L. Verdc. (İzmir Ivy) in vitro meristematic nodule regeneration potential in liquid cultures was investigated. In vitro sterile plantlets were obtained in semi-solid MS medium after surface sterilization of the seeds provided. 100% in MS-based K1, K5 and K6 stem explants for callus regeneration; K5 gave the best results with 100% in leaf explants. It was determined that this callus type is more suitable for liquid cultures, since leaf green callus are more loose. The highest percentage of meristematic nodule formation was obtained as 100% and the average fresh weight was 2.06 g in S33Z (½ MSB5 containing 4 mg/L BAP) nutrient medium. The blackening problem was eliminated by adding 100 mg/L Ascorbic acid. The highest biomass increase was recorded in ½ MSB5-based S33Z containing 100 mg/L L-Glutamine, 100 mg/L Ascorbic acid, coded as (Ç3), with a weekly average of 0.37 times. The highest SCV value (7.67%) was reached at the end of the 2nd week. In the study, after the meristematic nodules formation from leaf callus and secondary propagation, scale-up studies in nodule cultures of Vigna caracalla were optimized. In large-scale trials, Ç3 was used, when the effect of the inoculum was examined, 6 g/L meristematic nodule inoculum led to an increase in weekly average biomass of 0.52 times and the highest average fresh weight (27.19 g). It was determined that the harvested meristematic nodules converted into callus when cultured in Ç3 with different concentrations of BAP, GA3 and NAA added for regeneration experiments.