DETECTION OF THE FREQUENCY AND SUBTYPES OF BLASTOCYSTIS SP. IN PATIENTS WITH CHRONIC SPONTANEOUS URTICARIA
Project no. 74 · Status: Kapanmış · 2016
Summary
Blastocystis sp. is a common parasite of humans with controversial reports about its pathogenicity. Blastocystis sp. has a wide genetic diversity and can be detected different genotypes in patients with symptomatic or asymptomatic. Although this parasite often causes gastrointestinal complaints, it is known to cause urticaria in some patients. The aim of this study was to determine the frequency of Blastocystis sp. in patients with chronic spontaneous urticaria, to determine whether the cause of the symptoms was associated with Blastocystis sp. in patients with urticaria, and which Blastocystis sp. subtypes caused the urticaria by genotyping. In our study, 189 people (135 with chronic spontaneous urticaria, 54 with control group) were included in the study. To investigate Blastocystis and other parasites, it was examined by native-lugol method and cultured on Jones medium. Enzyme immunoassay (EIA) method was used to detect Cryptosporidium sp. and viruses (Adenovirus, Rotavirus) in order to determine whether there is coinfection in positive samples. DNA was isolated from each stool sample, followed by polymerase chain reaction (PCR) and sequencing methods. Blastocystis sp. positive was detected in 31.9% (43/135) patients in chronic urticaria and 14.8% (8/54) in the control group. In the patient group we examined, Giardia intestinalis was detected in 2 samples and Iodamoeba bütschlii in 1 sample. However, Cryptosporidium sp., Adenovirus and Rotavirus investigated by EIA were not detected. When the patient group and the control group were compared in terms of Blastocystis sp. positivity, it was found statistically significant (p <0.018). Thus, it has been revealed that there is a relationship between chronic urticaria and Blastocystis sp. Considering the subtype (ST) distribution of the patient group, ST3 (39.5%) was the most common. Other subtypes were ST 2 (23.2%), ST 1 (16.2%) and ST 7 (6.9%), respectively. This study Turkey microscopy, culture and molecular methods, and Blastocystis sp. prevalence of chronic urticaria patients is important in terms of a study investigating the relationship between this parasite and urticaria of both genotypes.