Investigation of the neuroprotective effect of graphene-based biomaterials and alpha (α)-lipoic acid in in vitro spinal cord injury models
Project no. 22125 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>Spinal cord injury (SCI) is a crucial clinical problem for which effective treatment has not yet been found. Although efforts are being made to restore spinal cord functions, especially after a partial or complete injury, it is still not possible to achieve complete regeneration following spinal cord injury. This study aimed to investigate the neuroprotective effects of graphene-doped biomaterials and alfa (α)-lipoic acid in in vitro mechanical and chemical spinal cord injury models on NSC-34 motor-neuron like cell line. Neuroblastoma × spinal cord hybrid cell line NSC-34 cells were applied to wells. A plastic pipette tip and/or chemical using 70 µM alfa (α)-kainic acid were used to induce mechanical (scratch model) and/or chemical spinal cord injury. MTT test was used to show cell viability; superoxide dismutase (SOD), glutathione S transferase (GST) and lactate dehydrogenase (LDH) tests were performed to show oxidative stress; synatptophysin, choline acetyltransferase and neurofilament immunofluorescent stainings were used to show cell morphology. A neuroprotective effect was observed with 0.05% graphene starting from day 1; alfa (α)-lipoic acid sodium bicarbonate salt provided a neuroprotective effect in long-term exposure (starting from day 9). The results of this study suggest graphene biomaterials and alpha (α)-lipoic acid may contribute to recovery following spinal cord injury.</p>