Synthesis of Photon Sensitive Nanofiber-Metal Controlled Drug Release Systems and Investigation of Their Activities
Project no. 23966 · Status: Kapanmış · 2022
Summary
<p>In order to reduce the side effects and increase the efficacy of Docetaxel (Doc), one of the most common chemotherapy drugs used in the treatment of breast cancer in women worldwide, the preparation of Docetaxel, Heparin (HP) and Fe3O4 nanoparticles loaded PLA:Gel bicomponent core:shell nanofibers was aimed in this thesis study. The preparation conditions were optimized by electrospinning method and the flow rate was determined as 0.4 ml/h for PLA, 1.2 ml/h for Gel, voltage as 10 kV and distance to the collector as 10 cm. In vitro release studies were carried out at 37 °C in pH 3, 5.5 and 7.4 environments by loading 2, 3 and 5% Doc (core part of the nanofiber) and HP (shell part of the nanofiber) active ingredients mono and dually onto 45:55% PLA:Gel nanofibers. It was observed that Dozetaxel in the core of the nanofibers was released slowly over a long period of approximately 80 hours, whereas the HP active substance in the shell section reached its maximum release capacity in approximately 10 hours. It was determined that the drug release of the dual drug-loaded (DocHP) nanofibers was similar to the single drug-loaded nanofibers and that more active substance could be released as the drug loading percentage in the nanofiber structure increased. In order to control the drug release profiles of the nanofibers with radiofrequency (RF), 30% Fe3O4 nanoparticles by mass were loaded into the core and shell parts of the nanofiber to the Doc and HP-loaded nanofiber structure. Doc and HP releases were examined by applying RF for 15 min and it was determined that the release rates of the active drug substances in their structures increased with the heating of the nanofibers as a result of RF application. viii The toxicity of the prepared drug-loaded 45:55% PLA:Gel nanofibers on MCF-7 breast cancer cells was determined and it was concluded that 2 mg 5% DocHP-loaded nanofiber inhibited the most cancer cells at the end of 72 hours.</p>