PRODUCING THE MIXED CULTURE ADAPTED FOR THE WASTEWATER TREATMENT AS A DRY MIXTURE USING VARIOUS CARRIERS AND DETERMINATING THEIR PRODUCT EFFICIENCY
Project no. 20014 · Status: Kapanmış · 2018
Summary
The demands and needs on the wastewater treatment system have increased significantly over the years in the subjects of removal of inorganic nitrogen and phosphorus, reduction of organic carbon, pathogen removal, breakdown of difficult-tobreak xenobiotic compounds. The formation of a microbial community which is capable of effective xenobiotic removal is often impaired by slow or poor adaptation of a slurry present to the compound. This situation is accompanied by impaired removal of N-, P- and C- and sludge settling problems. Within the community, bioagumentation which means creating new metabolic functions by adding bacteria or genetic information; is a possible method for dealing with these problems. With this study, an adapted, highly effective and high performance inoculation of culture with the degradation capacity of p-toluic acid (p-TOL), 4-carboxybenzaldehyde (4-CBA) and terephthalic acid (TPA) which are found in petrochemical industry wastewater was created, and thereafter in the reactor systems reproduction was carried out in the presence of economic substrates. It is aimed to determine the lyophilization and different storage conditions of the replicated bacterial cultures so that the lysis and viability activity will be optimal. Storage conditions are compared as +4°C and +27°C. At the same time, non-vacuum storage trials were conducted after lyophilization. This reproducted mixed culture is lyophilized with specific carriers under optimal conditions. As carriers, plant materials Medicago sativa, Glycine max and Helianthus annuus, which are not lignocellulosic natural starch components, were used. Carriers were matched with different preservatives and different dry mix product contents were created. Biodegradation and viability activity of the obtained product was determined for 18 months. The best products have been tested in an activated sludge system in a continuous mixing tank reactor (CSTR). On the CSTR scale, TPA, p-TOL and 4-CBA degradation were evaluated with total chemical concentrations of the highest 1200 mg/L. The selected microorganisms in the product were monitored by real-time PCR in the CSTR. Molecular results showed that Comamonas, Acinetobacter, Chryseobacterium and Raoutella were the dominant strains in the top product reactor. The content of +4°C “clover” is the group that preserves its vitality at the highest level among the contents used in the thesis study. When we look at the best HPLC results at +4°C, 100% removal of TPA (40 mg / L), p-TOL (40 mg / L) and 4-CBA (30 mg / L) within 4-7 hours, “clover”, “sunflower meal + skimmilk” and “soybean meal” product contents. At +27°C, the best group was "clover + silica" content. In addition, ecotoxicological evaluations were made on the basis of Microtox (Modern Water M500) toxicity measurement device data. When the ecotoxicological results of the metabolites whic are formed as a result of biodegradation activity, were evaluated, it was found that they were below the EC50 threshold level and were not toxic. In this way, inoculation cultures with long shelf life that can be commercialized were obtained.