The Role of PRP (Platelet Rich Plasma) in Preventing Intestinal Ischemia Reperfusion Injury in Rats

Project no. 31872 · Status: Kapanmış (Yayın bekleniyor) · 2023

Summary

Objective: Intestinal ischemia-reperfusion (I-R) injury is an important problem for surgeons in daily practice, and the processstarting with cellulardamage can lead to multi-organ failure. Platelet-rich plasma (PRP) is known to accelerate tissue healing and regulate inflammation due to its many growth factors and immunomodulators. However, there are not enough scientific studies on its role in the prevention of intestinal I/R injury. In our study, we aimed to investigate the biochemical, histological and immunohistochemical effectsof PRP on intestinal I/R injury induced in a rat model.Materials and Methods: In our study, four experimental groups were established using Wistar Albino rats: Sham, ischemia-reperfusion (I-R), PRP and I-R + PRP groups. In the I-R model, the SMA was clamped for 45 minutesfollowed by 120 minutes of reperfusion. PRP was administered intra-arterially to the groups prior to reperfusion. At the end of the experiment, WBC, neutrophil, hemoglobin, hematocrit and LDH levels in whole blood samples and SOD, MPO, MDA, TAS, TOS and OSI levels in plasma samples were evaluated by biochemical analysis. In addition, small intestinal tissues were subjected to histopathological examination and evaluated by immunohistochemical methods such as VEGF, iNOS, NF-κB.Results: Significant differences were found in all inflammatory and oxidative stress markers betweenthe I-R group and the sham group (p<0.05). Althoughthere was a decrease in neutrophil, OSI and MDA levels after PRP administration compared to the I-R group, this decrease was not statistically significant. On histopathological examination, mucosal integrity was better preserved in the PRP group, but there was no significant benefit in correcting I-R damage. Immunohistochemical staining showed that PRP applied after I-R decreased VEGF, iNOS, NF-κB levels, but this decrease was not statistically significant.Conclusion: Although PRP has an effect on tissue healing, oxidative stress and inflammatory response, we could not demonstrate its efficacy on intestinal I-R in acute condition. Further studies are needed to determine the protective and therapeutic efficacy of PRP in the long term and with other application methods.

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