A PLANT-DERIVED NANOPET AGENT (64CU-ENL-PLGA-PEG-DOTA): INVESTIGATION OF PROSTATE CANCER IMAGING POTENTIAL BY IN VITRO METHODS

Project no. 23245 · Status: Kapanmış (Yayın bekleniyor) · 2022

Summary

The potential of nanotechnology and plant-derived agents in combating cancer has garnered significant attention in the field of health sciences in recent years. The focus of research in this area is to find innovative solutions, particularly in the diagnosis and treatment of hormone-sensitive cancer types. Phytoöstrogens derived from flaxseeds, in particular, have been shown to exhibit positive effects against hormone-sensitive cancer types such as prostate cancer. Studies in the literature have demonstrated that these plant-derived compounds can inhibit the growth of cancer cells and even initiate the process of apoptosis (programmed cell death). Within the scope of this project, the aim is to formulate nanostructures from components obtained from flaxseeds and bacteria, and to label them with copper-64 (64Cu) radioisotope.This study comprehensively examined the cellular uptake and surface binding processes of 64Cu-ENLcom-NOTA and 64Cu-ENLbac-NOTA in prostate cancer cell lines, providing significant insights into the biological activity and interactions of these two molecules with cells. Notably, in LNCaP cells, both compounds exhibited a time-dependent increase in internalization, which was found to be sensitive to temperature. It was determined that 64Cu-ENLbac-NOTA exhibited stronger surface binding and higher cellular uptake compared to 64Cu-ENLcom-NOTA. However, in PC3 and RWPE-1 cell lines, internalization was found to be independent of temperature and remained minimal. These findings indicate that both molecules show high cell retention in prostate cancer cell lines and hold potential as PET imaging agents, with the necessary in vitro data having been obtained to proceed to in vivo animal studies.Keywords: Phytoestrogens, Pozitron Emission Tomography (PET), Copper-64 (64Cu), Prostate Cancer.

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