Determination of the antidiabetic effect of Sarcopoterium spinosum (Abdestbozan) plant in chicken embryo model
Project no. 21822 · Status: Kapanmış (Yayın bekleniyor) · 2020
Summary
<p>Diabetes is a metabolic and chronic disease that affects 537 million people worldwide and can cause serious complications and diseases with increasing mortality every year. Medicinal plants and bioactive compounds isolated from plants have been the source of antidiabetic drugs in the treatment of type 2 diabetes (T2D) due to the high treatment costs and side effects of synthetic drugs available in the treatment of diabetes. The plant <em>Sarcopoterium spinosum</em> (<em>S. spinosum</em>), a thorny dwarf shrub, and its bioactive compounds play an important role in the treatment of diabetes. In addition to <em>in vivo</em> and <em>in vitro</em> models, the development of the chicken embryo model as <em>in ovo</em> model to examine and correlate diabetes and diabetes-related complications with existing models will accelerate the process of examining the antidiabetic effects of plant extracts and their bioactive compounds.</p><p>In this study, the antidiabetic activity of the ethanolic extracts obtained by the optimization of the extraction of the leaf and root parts of the <em>S. spinosum</em> plant and the bioactive pure compounds isolated from the extract by preparative thin layer chromatography (Prep TLC) were investigated with a chicken embryo model. The effect on blood glucose and insulin levels was compared with the metformin-treated group and control group results. The plant extracts and LC-MS analysis resulted in a significant decrease in blood glucose levels of diabetic chicken embryos in the groups treated with bioactive compounds, α-tocopherol and epicatechin, comparable to the metformin-treated group. As a result, extracts obtained from the root and leaf parts of <em>S. spinosum</em> plant and isolated α-tocopherol and epicatechin bioactive compounds showed antidiabetic activity by regulating blood sugar, insulin levels and lipid profiles in diabetic chicken embryos with the <em>in ovo</em> model system.</p>