The Effects of Differently Processed Poppy and Ladania Pollens on the Midgut Histology of the Efe Bee (Apis mellifera anatoliaca)

Project no. 32640 · Status: Kapanmış (Yayın bekleniyor) · 2024

Summary

<p>In&nbsp;this&nbsp;study,&nbsp;the&nbsp;effects&nbsp;of&nbsp;differently&nbsp;processed&nbsp;poppy&nbsp;(Papaver&nbsp;somniferum)&nbsp;and&nbsp;ladania&nbsp;(Cistus&nbsp;creticus)&nbsp;pollens&nbsp;on&nbsp;the&nbsp;midgut&nbsp;histology&nbsp;of&nbsp;Efe&nbsp;bee&nbsp;(Apis&nbsp;mellifera&nbsp;anatoliaca)&nbsp;were&nbsp;investigated&nbsp;for&nbsp;the&nbsp;first&nbsp;time.&nbsp;The&nbsp;worker&nbsp;bees&nbsp;kept&nbsp;in&nbsp;a&nbsp;total&nbsp;of&nbsp;27&nbsp;cages&nbsp;were&nbsp;fed&nbsp;with&nbsp;fresh,&nbsp;pre-treated,&nbsp;and&nbsp;fermented&nbsp;poppy,&nbsp;ladania,&nbsp;and&nbsp;mixed&nbsp;(%50&nbsp;poppy+%50&nbsp;ladania)&nbsp;pollens&nbsp;for&nbsp;14&nbsp;days,&nbsp;and&nbsp;the&nbsp;midgut&nbsp;inner&nbsp;and&nbsp;outer&nbsp;diameters,&nbsp;epithelial&nbsp;thickness,&nbsp;and&nbsp;digestive&nbsp;cell&nbsp;counts&nbsp;were&nbsp;evaluated.&nbsp;The&nbsp;significant&nbsp;increase&nbsp;in&nbsp;midgut&nbsp;diameter&nbsp;in&nbsp;bees&nbsp;fed&nbsp;with&nbsp;fermented&nbsp;poppy&nbsp;pollen&nbsp;compared&nbsp;to&nbsp;those&nbsp;fed&nbsp;with&nbsp;fresh&nbsp;poppy&nbsp;pollen&nbsp;suggested&nbsp;that&nbsp;fermentation&nbsp;supports&nbsp;the&nbsp;digestion&nbsp;and&nbsp;utilization&nbsp;of&nbsp;nutrients.&nbsp;In&nbsp;ladania&nbsp;pollen,&nbsp;the&nbsp;effect&nbsp;of&nbsp;fermentation&nbsp;was&nbsp;particularly&nbsp;evident&nbsp;at&nbsp;the&nbsp;cellular&nbsp;level,&nbsp;the&nbsp;number&nbsp;of&nbsp;digestive&nbsp;cells&nbsp;in&nbsp;the&nbsp;fermented&nbsp;ladania&nbsp;pollen&nbsp;decreased&nbsp;significantly&nbsp;compared&nbsp;to&nbsp;the&nbsp;fresh&nbsp;ladania&nbsp;pollen.&nbsp;This&nbsp;finding&nbsp;was&nbsp;associated&nbsp;with&nbsp;the&nbsp;disruption&nbsp;of&nbsp;the&nbsp;pollen&nbsp;wall&nbsp;by&nbsp;fermentation&nbsp;and&nbsp;the&nbsp;subsequent&nbsp;facilitation&nbsp;of&nbsp;nutrient&nbsp;absorption.&nbsp;The&nbsp;fact&nbsp;that&nbsp;the&nbsp;epithelial&nbsp;thickness&nbsp;was&nbsp;unaffected&nbsp;by&nbsp;the&nbsp;treatments&nbsp;in&nbsp;both&nbsp;the&nbsp;pollen&nbsp;groups,&nbsp;indicated&nbsp;that&nbsp;fermentation&nbsp;and&nbsp;physical&nbsp;pre-treatments&nbsp;did&nbsp;not&nbsp;cause&nbsp;any&nbsp;distinct&nbsp;pathological&nbsp;changes&nbsp;in&nbsp;the&nbsp;ventriculus.&nbsp;The&nbsp;effect&nbsp;of&nbsp;fermentation&nbsp;was&nbsp;demonstrated&nbsp;by&nbsp;an&nbsp;increase&nbsp;in&nbsp;midgut&nbsp;diameter&nbsp;in&nbsp;the&nbsp;poppy&nbsp;pollen&nbsp;and&nbsp;a&nbsp;decrease&nbsp;in&nbsp;digestive&nbsp;cell&nbsp;count&nbsp;in&nbsp;ladania&nbsp;pollen.&nbsp;Consequently,&nbsp;instead&nbsp;of&nbsp;feeding&nbsp;honeybees&nbsp;pollens&nbsp;in&nbsp;their&nbsp;fresh&nbsp;form,&nbsp;administering&nbsp;fermented&nbsp;poppy&nbsp;and&nbsp;ladania&nbsp;pollens&nbsp;is&nbsp;recommended,&nbsp;particularly&nbsp;to&nbsp;protect&nbsp;the&nbsp;digestive&nbsp;health&nbsp;of&nbsp;the&nbsp;Efe&nbsp;bee,&nbsp;accelerate&nbsp;colony&nbsp;development,&nbsp;and&nbsp;enhance&nbsp;bioavailability.</p>

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