Investigation of epidermis histologic structure of two leeches species Hirudo sulukii and Hirudo verbana (Hirudinida: Hirudinidae)
Project no. 20862 · Status: Kapanmış · 2019
Summary
In this study, it was aimed to investigate the staining properties of secretory cells via histochemical stains and determine secretory cell types and epidermis histology in the body wall of important medicinal leeches called Hirudo verbana and Hirudo sulukii. In addition, areas of epidermis epithelial cells, areas of secretory cell types and secretory areas of secretory cells stained with histochemical stains (Periodic Acid Schiff(PAS) and Alcian Blue(AB)) were statistically compared in both species. The cuticle layer is located on the epidermis consisting of simple monolayer cylindrical epithelium and secretory cells. From some Type 1 cells located close to the epidermis were determined as pear-shaped secretory cells. Type 2a and Type 2b cells are located in groups starting from the dermis towards the inner sides of the body wall. While the secretory cells close to the epidermis were stained weak with PAS and AB, Type 2a cells were darker and Type 2b secretory cells were the darkest cells. The striated muscle layer is located circular, longitudinally and obliquely. Statistical calculations showed that areas of epithelial and secretory cells are generally larger in H. sulukii than H. verbana. Therefore, it is considered that H. sulukii is more resistant species than H. verbana. Since both PAS and AB staining methods showed that secretory areas of secretory cells were larger in H. sulukii, we concluded that mucous composition between two species was different concentration.