EVALUATION FOR VIRULENCE AND COPPER TOLERANCE OF Xanthomonas arboricola pv. juglandis STRAINS THE CAUSAL AGENT OF BACTERIAL BLIGHT
Project no. 1034 · Status: Kapanmış · 2016
Summary
Bacterial blight of Persian walnut (Juglans regia), caused by Xanthomonas arboricola pv. juglandis (XAJ) and, Brown Apical Necrosis (BAN) caused by XAJ with saprophytic fungi result premature fruit drops if measures are not taken in walnut growing areas. In this study; XAJ strains, which were previously isolated from different walnut growing regions, and stored in culture collection of Bacteriology Laboratory of the Faculty of Agriculture of Ege University, were tested in terms of virulence and copper tolerance. According to the results of in vitro tests, it was determined that the most tolerant XAJ strains to copper obtained 0,8 mM to1 mM MIC values and the most sensitive XAJ strains to Copper obtained 0,1 mM MIC values. In vitro and In vivo tests results were confirmed each other when XAJ strains which were selected as tolerant and susceptible to copper depending on in vitro test results. However, it has been determined that the virulence of XAJ strains which showed tolerance to copper was low. XAJ strains identified as tolerant and susceptible to copper were selected and tested by PCR for the presence of the copA and copB genes which were responsible from copper tolerance, but, these genes were not detected by molecular ways.