Critical time point for apoptotic cell death in an experimental ischemia/reperfusion model and the effect of N-acetylcystein
Makaleler > Tam Makale · 2017
Yazarlar: Gönen ÖZŞARLAK SÖZER, MUSTAFA EMRE, Serhat DEMİRKOL, ARBİL AÇIKALIN, SALİH ÇETİNER, Zeki TOPÇU, Devrim Demir-Dora
Turkish Journal of Biochemistry, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2017, cilt 42, ss 265-272
Özet
Abstract Objective Kidney transplantation is an important treatment option in end stage renal failure. Tissue death may be an important problem when a kidney is removed from a cadaver and transported to a donor a long distance away. The purpose of this study is to determine the critical time point for apoptotic cell death in a renal ischemia/reperfusion model and determine the effects of N-acetylcystein on apoptosis induced by ischemia injury. Methods Apoptotic cell death after induced renal ischemia followed by reperfusion, was estimated in a group of Wistar albino rats by immunoflourescence and ELISA techniques. N-acetylcystein, an antioxidant agent, was given to the rats to study the effect on apoptosis. Tissues were examined immunohistochemically at 0, 1 h, 24 h, 5 days and 10 days for detection of apoptotic cells. Results Our results showed that an ischemia for 60 min followed by reperfusion for 60 min triggered apoptosis. Moreover, N-acetylcystein significantly diminished both the ischemia/reperfusion damage and apoptosis. Conclusion We anticipate our results would be important for kidney transplantation in estimating the critical time point for apoptosis and administration of N-acetylcystein prior to removal of the organ may be important in delaying the onset of apoptosis.