Metabolomics profiling, biological activities, and molecular docking studies of elephant garlic (Allium ampeloprasum L.)

Ceren EMİR; Güneş ÇOBAN; Ahmet EMİR

doi:10.1016/j.procbio.2022.03.002

Metabolomics profiling, biological activities, and molecular docking studies of elephant garlic (Allium ampeloprasum L.)

Makaleler > Tam Makale · 2022

Yazarlar: Ceren EMİR, Güneş ÇOBAN, Ahmet EMİR

Process Biochemistry, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2022, cilt 116, ss 49-59

DOI: 10.1016/j.procbio.2022.03.002

Özet

<span style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"><a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/allium" title="Learn more about Allium from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">Allium</a> ampeloprasum<span style="color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"> L., elephant garlic, is a plant that is cultivated and notable for its usage for medical and nutritional purposes. From this point, the present work presents the phenolic profiles of methanol extracts from the flowers, leaves, and bulbs of <em style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;">A. ampeloprasum<span style="color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"> collected in the wild in Izmir, Turkey, using a liquid chromatography-mass spectrometry technique. Among the 30 quantified phenolic compounds, 3-hydroxybenzoic acid was the major component in <em style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;">A. ampeloprasum<span style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"> from Cambel (AA2) with a value of 3505 ± 12.8 µg/g extract, while the bioactive flavonols, <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/quercetin" title="Learn more about quercetin from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">quercetin</a> (98.37 ± 1.75 µg/g extract), and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/kaempferol" title="Learn more about kaempferol from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">kaempferol</a> (173.20 ± 1.3 µg/g extract) were notable molecules in samples of <em style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;">A. ampeloprasum<span style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"> from Armutlu (AA1). Additionally, <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/antioxidant-activity" title="Learn more about antioxidant activity from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">antioxidant activity</a> by ferric-reducing antioxidant power and cupric ion-reducing assays, and the total phenolic, and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/flavonoid" title="Learn more about flavonoid from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">flavonoid</a> content were determined for all extracts. Finally, the samples’ capacity to inhibit the enzymes <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/acetylcholinesterase" title="Learn more about acetylcholinesterase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">acetylcholinesterase</a>, <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/butyrylcholinesterase" title="Learn more about butyrylcholinesterase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">butyrylcholinesterase</a>, and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/tyrosinase" title="Learn more about tyrosinase from ScienceDirect's AI-generated Topic Pages" class="topic-link" style="margin: 0px; padding: 0px; text-decoration-line: underline; text-decoration-thickness: 1px; text-decoration-color: rgb(46, 46, 46); color: rgb(46, 46, 46); word-break: break-word; text-underline-offset: 1px;">tyrosinase</a> was evaluated using a 96-well microplate reader, the flowers of both samples generally had stronger inhibitory activity. Furthermore, the relationship between enzyme inhibition potency and the main phenolic components was expounded by molecular modeling studies. Consequently, these experiments were achieved to investigate the prospective usage of <em style="margin: 0px; padding: 0px; color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;">A. ampeloprasum<span style="color: rgb(46, 46, 46); font-family: NexusSerif, Georgia, "Times New Roman", Times, STIXGeneral, "Cambria Math", "Lucida Sans Unicode", "Microsoft Sans Serif", "Segoe UI Symbol", "Arial Unicode MS", serif; font-size: 18px;"> both in pharmaceutical and nutraceutical fields.

Detay görüntüle