Amine intercalated clay surfaces for microbial cell immobilization and biosensing applications
Articles > Article · 2013
Authors: Bilal DEMIR, Muharrem SELECI, Didem AG, Serdar Cevik, Esra Evrim YALÇINKAYA, Dilek ODACI, Ulku Anik, Suna TİMUR
RSC Advances, Journal Indexed in SCI Expanded, International, WoS, 2013, vol 3, pp 7513-7513
DOI: 10.1039/c3ra40335a
Summary
Trimethylamine (TM) intercalated montmorillonite (Mont) clay was prepared and characterized using X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric measurements. Gluconobacter oxydans cells were immobilized on the micro-structured matrix and used in microbial sensor applications. Sensor responses were based on the respiratory activity of the cells and the consumption of oxygen was monitored at −0.7 V (vs. Ag/AgCl and platinum electrodes) by using glucose as a substrate. Stabilization of the bacteria was performed on the Mont using glutaraldehyde. Optimization of the TM-Mont/G. oxydans sensor and examination of the electrochemical mechanism were carried out in a batch system. Measurements concerning analytical characteristics, operational stability, repeatability and substrate specificity depending on the carbon source in the culture were investigated in a flow injection analysis (FIA) system. Linear ranges were found between 0.15 and 5.0 mM for the batch mode and 0.1 and 5.0 mM for the FIA system, respectively. Finally, real samples were analyzed and were compared with the results of a spectrophotometric method as reference.