Arginine Selective Biosensor Based on Arginase‐Urease Immobilized in Gelatin
Makaleler > Tam Makale · 2003
Yazarlar: Selma Karacaoğlu, Suna Timur, Azmi Telefoncu
Artificial Cells, Blood Substitutes, and Biotechnology, Uluslararası, Wos, 2003, cilt 31, ss 357-363
Özet
Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross-linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris-HCl buffer (pH 8.5) was used at 25 degrees C. The biosensor response depends linearly on arginine concentration between 0.025-0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.