Annexin‐1 as a salivary biomarker for gingivitis during pregnancy
Makaleler > Tam Makale · 2018
Yazarlar: Manar N. Hassan, Georgios N. Belibasakis, Pınar MERİÇ KANTAR, Veli Ozgen Ozturk, Gülnur EMİNGİL, Nagihan Bostanci
Journal of Periodontology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2018, cilt 89, ss 875-882
DOI: 10.1002/jper.17-0557
Özet
BACKGROUND: It is well established that there is higher susceptibility to gingival inflammation during pregnancy. Annexin-1 (ANXA1) is an anti-inflammatory protein which has been identified in gingival tissue exudates by discovery proteomics. This cross-sectional case-control study investigated the levels and association of ANXA1 and pro-inflammatory mediator interleukin (IL)-1β in the saliva of pregnant and non-pregnant women. METHODS: Whole unstimulated saliva from 69 non-pregnant and 78 pregnant women was collected prior to measurement of probing depth, clinical attachment level, bleeding on probing, and plaque. Then, the women were split into 3 subgroups depending on their periodontal status (healthy, gingivitis, and periodontitis). The levels of ANXA1 and IL-1β were measured with enzyme-linked immunosorbent assay and reported as pg/mg after normalizing against the total protein levels. RESULTS: Significantly higher ANXA1 levels were exhibited in pregnant women with gingivitis compared with health (P < 0.05) and in pregnant women with gingivitis compared with the respective non-pregnant group (P < 0.0001). There was a significantly higher level of IL-1β in gingivitis than in health in pregnant women (P < 0.05) and significantly higher levels in periodontitis compared with health in non-pregnant women (P < 0.05). Looking at the IL-1 β:ANXA1 ratio, the non-pregnant periodontitis group displayed a significantly higher ratio compared with the respective pregnant group (P < 0.05). In the non-pregnant subpopulation, the ratio was significantly higher in periodontitis compared with health (P < 0.01). CONCLUSION: Salivary ANXA1 levels are elevated in the presence of gingivitis only in pregnant, but not non-pregnant women, rendering this molecule as a potential salivary biomarker for non-invasive early screening for gingival inflammation during pregnancy.