Use of Eggshell Membrane as an Immobilization Platform in Microbial Sensing
Articles > Article · 2008
Authors: Fatma YENİ, Dilek ODACI, Suna TİMUR
ANALYTICAL LETTERS, Journal Indexed in SCI Expanded, International, 2008, vol 41, pp 2743-2758
DOI: 10.1080/00032710802363594
Summary
A new microbial biosensor was developed by immobilizing Pseudomonas fluorescens cells on eggshell membrane via physical adsorbtion. Bacteria-modified eggshell membrane was fixed tightly onto the surface of a carbon paste electrode (CPE) with a silicone rubber o-ring to construct a microbial biosensor. The measurements were based on the respiratory activity of the cells caused by oxygen consumption during bacterial metabolism. A mediated biosensor was also developed by ferrocene. As well as the response characteristics, stabilities and substrate specificities were investigated. Data were given as the comparison of mediated and unmediated systems.