Immobilization of laccase for biotechnology applications
Articles > Article · 2013
Authors: Şenay ŞANLIER, Simge GIDER, Alper KOPRULU
Artificial Cells, Nanomedicine, and Biotechnology, Journal Indexed in SCI Expanded, International, Scopus, 2013, vol 41, pp 259-263
DOI: 10.3109/10731199.2012.731414
Summary
Laccase played an important role in the decolorization of wide spectrum dyes as a low-cost and environmentally friendly technology. Laccase was immobilized in alginate beads and immobilization conditions were identified. 25 mg/ml laccase enzyme encapsulation efficiencies of using the prepared bead was calculated as approximately 94%. At the end of the 10 days of storage, the free laccase and immobilized laccase retained about 8.08% and 80.83%, respectively. The decolorization of the dye (Direct Blue 2) was around 86% for immobilized enzyme at 45°C. In the study, compared to the free enzyme, high activity, stable, reusable immobilized enzyme preparation was prepared.