Egg white protein polymer: an affinity matrix for protease enrichment and isolation
Articles > Article · 2021
Authors: Esra Menfaatli, Figen ZİHNİOĞLU
Separation Science and Technology, Journal Indexed in SCI Expanded, International, WoS, 2021, vol 57, pp 103-111
DOI: 10.1080/01496395.2021.1887235
Summary
Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE results and diffusion agar plate tests supported the protease-binding efficiency of the polymer. In conclusion, egg white protein polymer can specifically bind proteases and it is a convenient and cost-effective material for enrichment, isolation, and removal of proteases.