Egg white protein polymer: an affinity matrix for protease enrichment and isolation
Makaleler > Tam Makale · 2021
Yazarlar: Esra Menfaatli, Figen ZİHNİOĞLU
Separation Science and Technology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2021, cilt 57, ss 103-111
DOI: 10.1080/01496395.2021.1887235
Özet
Egg white was precipitated with acetone and cross-linked with glutaraldehyde. Protease specificity of the polymer was investigated with different types of proteases and non-protease proteins. Protein polymer effectively bound trypsin, papain and the different proteases from pancreatin. SDS-PAGE results and diffusion agar plate tests supported the protease-binding efficiency of the polymer. In conclusion, egg white protein polymer can specifically bind proteases and it is a convenient and cost-effective material for enrichment, isolation, and removal of proteases.