Purification of α-Galactosidase by Affinity Precipitation with Alginate
Makaleler > Tam Makale · 2008
Yazarlar: Evran Bicak Celem, Seçil ÖNAL
Preparative Biochemistry & Biotechnology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2008, cilt 38, ss 348-357
DOI: 10.1080/10826060802325436
Özet
Affinity precipitation is a technique which is known for over 20 years, but has recently received more attention due to the development of new materials for its implementation. It is a relatively simple, convenient, and reproducible technique that results in high target molecule recovery at high specificity. We describe, here, an efficient and rapid purification procedure for Vicia faba alpha-galactosidase (EC 3.2.1.22) by using affinity precipitation with alginate. The enzyme was purified with 43% activity yield and 40-fold purification. SDS-PAGE of the purified enzyme showed a single band and a subunit weight of 44 kDa. The properties of the enzyme were also searched. The results showed that the general properties of the enzyme offer potential for use of this alpha-galactosidase in several production processes.