Homocysteine effects on cellular glutathione peroxidase (GPx-1) activity under in vitro conditions
Articles > Article · 2007
Authors: Ayşen DURMAZ GÜVEN, Nurten DİKMEN
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, Journal Indexed in SCI Expanded, International, 2007, vol 22, pp 733-738
DOI: 10.1080/14756360601164929
Summary
Hyperhomocysteinemia is associated with a lot of diseases including cardiovascular diseases and neural tube defect, but it has not been clarified exactly which mechanism is responsible for occurence disease. Here, homocysteine (Hcy) and cysteine (Cys), which are thiol containing amino acids, were examined for their effect on glutathione peroxidase (GPx) activity. It was observed that the GPx-1 activity was inhibited under severe hyperhomocysteinemia (50-500 mu M Hcy) conditions, especially at low glutathione concentrations but that cysteine increased GPx-1 activity at low glutathione concentrations and inhibition clearly appeared at 500mM Cys concentration.