Homocysteine effects on cellular glutathione peroxidase (GPx-1) activity under in vitro conditions
Makaleler > Tam Makale · 2007
Yazarlar: Ayşen DURMAZ GÜVEN, Nurten DİKMEN
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2007, cilt 22, ss 733-738
DOI: 10.1080/14756360601164929
Özet
Hyperhomocysteinemia is associated with a lot of diseases including cardiovascular diseases and neural tube defect, but it has not been clarified exactly which mechanism is responsible for occurence disease. Here, homocysteine (Hcy) and cysteine (Cys), which are thiol containing amino acids, were examined for their effect on glutathione peroxidase (GPx) activity. It was observed that the GPx-1 activity was inhibited under severe hyperhomocysteinemia (50-500 mu M Hcy) conditions, especially at low glutathione concentrations but that cysteine increased GPx-1 activity at low glutathione concentrations and inhibition clearly appeared at 500mM Cys concentration.