Bombyx mori (Lepidoptera: Bombycidae)
Makaleler > Tam Makale · 2015
Yazarlar: Selek, Gözde, Nesrin Ebru GÖNCÜ, Parlak, Osman
European Journal of Biology, Uluslararası, 2015
Özet
In silkworms, like other holometabolus insects, larval digestive canal degenerates via programmedcell death (PCD) during larval-pupal metamorphosis. PCD plays a key role in developmental biology.It generally occurs with two major mechanisms; apoptosis and autophagic cell death. In this study,we aimed to determine the metamorphic events in the digestive canal of Bombyx mori during theprepupal period. The general morphological changes were studied by using histological techniques; thedegenerative cells were determined using H&E (Hematoxylen&Eosin) and PAS (Periodic Acid Schiff)staining. We have used TUNEL (terminal transferase-mediated dUTP nick end-labeling) assay fordetermination of apoptotic cell nuclei. Also, the acid phosphatase activity was calculated to determinethe autophagic cell death. In conclusion; our observations indicated a co-occurrence of apoptosis andautophagy observed during the cell death of the digestive canal.