Investigation of large-scale production-optimized exosomes in a hepatocellular carcinoma model
Bildiriler > Özet Bildiri · 2023
Yazarlar: Ilgın Kımız Geboloğlu, Ayşe Gülten Kantarcı, Suphi Şurişvan ÖNCEL
Small New World 2.0, Avusturya, Graz, Uluslararası, Poster, Özet Bildiri, 2023-2023, cilt ., ss 18-.
Özet
<p>Membrane vesicles released from prokaryotic and eukaryotic cells, such as exosomes, microvesicles, and</p><p>apoptotic bodies, represent a dynamic extracellular vesicular domain that is increasingly recognized in basic</p><p>research and translational clinical development. Exosomes are nanovesicles 20-100 nm in size, called natural</p><p>liposomes. Research into the use of exosomes in therapeutic therapy as delivery systems has increased</p><p>rapidly since the discovery of exosomes in the 1970s. There is a lot of research focusing on its use not only in</p><p>oncology, but also in the treatment of cardiovascular diseases, autoimmune syndromes, neurodegenerative</p><p>diseases such as Alzheimer's and Parkinson's disease, and infections caused by viruses such as tuberculosis,</p><p>diphtheria, toxoplasma and HIV, and the delivery of drugs and vaccines. In this study, it is aimed to obtain a large</p><p>amount of exosomes for therapeutic purposes in a short time by producing exosomes in a controlled</p><p>bioreactor. For this purpose, the human monocyte cell line (THP-1) was adapted to a chemically defined</p><p>serum-free medium so that the exosomes, which are primarily intended to be used for therapeutic purposes,</p><p>do not cause cross-reaction. Then, the optimization of cell production in a stirred tank bioreactor, the</p><p>optimization of the ultrafiltration method, which is more suitable for large scale during exosome isolation from</p><p>cells, and the characterization of the exosomes are provided. Finally, the therapeutic effect of exosomes was</p><p>investigated in 3D in vitro hepatocellular carcinoma cell spheroid model.</p><p>Acknowledgments: This work was supported through research grants from Ege University Scientific Research Projects</p><p>Coordination with a project number of 22868.</p>