Protective Role of Some Vitamins Against DNA Damage: An Electrochemical Approach
Makaleler > Tam Makale · 2026
Yazarlar: Mihrican Muti, Kübra Gençdağ Şensoy, Pelin Uysal Çalıcıoğlu, Hakan KARADENİZ
Afyon Kocatepe University Journal of Sciences and Engineering, Uluslararası, 2026, cilt 26, ss 431-438
DOI: 10.35414/akufemubid.1671726
Özet
This study investigates the electrochemical detection of DNA damage induced by infrared (IR) radiation and evaluates the protective effects of vitamins C, D, E, and B7 (biotin) against such damage. DNA was immobilized onto a pencil graphite electrode (PGE), and variations in the guanine oxidation signal were monitored by differential pulse voltammetry (DPV) in 0.5 M acetate buffer (pH 4.8) using a three-electrode system (PGE/Ag–AgCl/Pt). DNA-modified electrodes (DNA–PGE) and vitamin-coated DNA–PGEs, prepared by incubating the DNA–PGEs in vitamin solutions, were exposed to IR radiation for 1 h. In the absence of vitamins, IR exposure caused a marked decrease in the guanine oxidation peak current from 1.55 µA to 0.67 µA (a 57% reduction), indicating substantial DNA damage. In contrast, electrodes coated with vitamins D and B7 exhibited statistically significant protection (p < 0.05), as evidenced by a significantly reduced decrease in the guanine oxidation signal following IR exposure. Vitamins C and E, however, did not show a significant protective effect. These findings suggest that vitamins D and B7 may serve as potential protective agents against IR-induced genotoxic damage.