A bio-imprinted ascorbate oxidase biosensor
Articles > Article · 2007
Authors: Ayse B. TEKE, M. Kemal SEZGINTURK, Mustafa TEKE, Erhan DİNÇKAYA, Azmi TELEFONCU
International Journal of Environmental Analytical Chemistry, Journal Indexed in SCI Expanded, International, WoS, 2007, vol 87, pp 723-729
DOI: 10.1080/03067310701327758
Summary
Interest in bio-imprinting techniques has increased because it allows some stability characteristics of enzymes to be improved. In this study, we developed a simple way to improve the thermal and pH stabilities of ascorbate oxidase biosensor. The membrane of a Clark oxygen electrode was coated by a bioactive layer containing ascorbate oxidase and gelatin cross-linked by glutaraldehyde. Citrate was used to imprint the ascorbate oxidase molecularly. The optimum temperature and pH of both unmodified and citrate modified biosensors were investigated, by comparing their resulting stability. Also, calibration graphs and operational stabilities were compared with each other. The results showed that this simple way should be used to improve the stabilities of a biosensor.