Self-assembling nanomaterial-based peptide surface for target cell adhesion
Makaleler > Tam Makale · 2023
Yazarlar: Hasret Turkmen
Journal of Electrochemical Science and Engineering, Uluslararası, Scopus, 2023, cilt 13, ss 491-504
DOI: 10.5599/jese.1664
Özet
Non-covalent modification of electrode surfaces with nanoparticle-based peptides does not change the chemical properties of the electrode but allows electrochemical measurement of cell adhesion. This study examines the effect of self-modified nanomaterial/peptide surfaces on cell adhesion. This adhesion to the surface is caused by the negative Gibs free energy formed in the system because of the presence of -0H, sulfur, carbonyl, or reactive groups. A cheaper and more practical method for electrode surfaces targeting cell adhesion, which does not use heavy chemicals and EDC/NHS chemistry, is used in this work. Thanks to the bioactive materials immobilized on the screen-printed carbon electrode (SPCE) surface in a controlled manner and the surface chemistry offered by these materials, a biocompatible self-assembling nanomaterial-based peptide surface platform is created, and cell adhesion is measured by an electrochemical technique. After the characterization steps, electrochemical techniques created a calibration curve of the current value as a function of concentration for each cell line. The adhesion of the generated bioactive electrode surfaces to the selected cell lines was examined comparatively.