The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid
Makaleler > Tam Makale · 2009
Yazarlar: Hakan KARADENİZ, Güliz ARMAGAN, Kadriye Arzum ERDEM GÜRSAN, Ezgi Turunc, Ayfer Çalışkan, Lütfiye KANIT, Ayfer YALÇIN
Electroanalysis, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2009, cilt 21, ss 2468-2476
Özet
Abstract A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA‐treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method.