QUANTITATIVE DETERMINATION OF GALANTHAMINE AND LYCORINE IN GALANTHUS FOSTERI
Makaleler > Tam Makale · 2012
Yazarlar: Gülen İrem KAYA, Ahmet EMİR, B Sarıkaya, M. A. Onur, Somer N. Unver
Reviews on Clinical Pharmacology and Drug Therapy, Uluslararası, 2012, cilt 10, ss 66-1
DOI: 10.17816/rcf10266_1
Özet
Among the 14 taxa and one hybrid of the genus Galanthus growing in Turkey, Galanthus fosteri baker is distributed mainly in south-and north-central Turkey (1, 2).Galanthamine, the most important alkaloid found in Amaryllidaceae species, is used for the treatmant of Alzheimer's disease (3).lycorine, a widespread alkaloid found in Amaryllidaceae plants has important biological activities (4).Therefore it is important to quantify these alkaloids in Amaryllidaceae species. in the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine and galanthamine in G. fosteri.The chromatographic separation was performed using an isocratic system with a mobil phase trifluoroacetic acid-water-acetonitrile (0.01: 92.5: 7.5) and diode array dedector (5).The linearity of the method was studied by injecting five known concentrations of lycorine in the range of 2.5-40 µg/ml and five known concentrations of galanthamine in the range of 1-16 µg/ml.The calibration curves for lycorine and galanthamine were determined as y = 15.6024x+0.4459and y = 10.8302x-1.9292,respectively.The contents of galanthamine in the aerial parts was found to be 0.003 % whereas the amount of the lycorine was found as 0.010 %.The contents of galanthamine and lycorine in the bulbs of G. fosteri were found to be 0.012 % and 0.035 %, respectively.