Calixarene modified montmorillonite: a novel design for biosensing applications
Articles > Article · 2014
Authors: Burak SONMEZ, Serkan Sayin, Esra Evrim YALÇINKAYA, Didem Ag SELECI, Huseyin Bekir Yildiz, Dilek ODACI, Suna TİMUR
RSC ADVANCES, Journal Indexed in SCI Expanded, International, 2014, vol 4, pp 62895-62902
DOI: 10.1039/c4ra11818a
Summary
Here we report the synthesis, characterization and application of calixarene (Calix) modified montmorillonite (Mt) as a platform for bio-applications such as biomolecule immobilization and biosensing technologies. This modification enhanced the biomolecule immobilization capability of Mt. Initially, amino-functionalised calixarenes (Calix-NH2) were synthesized and used as a modifier. X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric analysis were performed to verify the modification of the clay minerals. For the biosensor construction, Calix-NH2 modified Mt (Calix-NH2/Mt), bovine serum albumin (BSA), glutaraldehyde (GA) and pyranose oxidase were immobilized on the surface of a glassy carbon electrode which was then referred to as a CalixNH(2)/ Mt/PyOx biosensor. After optimization of the enzyme amount and pH, analytical characteristics were investigated in detail.