Screening of Y chromosome microdeletion which contains AZF regions in 71 Turkish azoospermic men.
Makaleler > Tam Makale · 2004
Yazarlar: O Okutman-Emonts, S Pehlivan, Erol TAVMERGEN, E N Tavmergen-Goker, Feriştah Ferda ÖZKINAY
Genetic counseling (Geneva, Switzerland), Uluslararası, 2004, cilt 15, ss 199-205
Özet
Screening of Y chromosome microdeletion which contains AZF regions in 71 turkish azoospermic men: In 71 Turkish men Y chromosome microdeletions have been studied before intracytoplasmic sperm injection (ICSI). DNA samples were amplified with 18 STS primers of the azoospermia factor (AZF) region on the Y chromosome by using multiplex polymerase chain reaction (PCR). Microdeletions were detected in 4 azoospermic men (5.6 %); one with a deletion in the AZFb region, while the 3 others had a large deletion extending over multiple chromosomal regions (AZFb+c+d and AZFa+b+c+d). In the patients with microdeletion, no spermatogenetic activity could be detected in testis biopsies. This result confirms the idea that Y chromosome microdeletion analysis is important in investigating the possibility of finding sperm in testicular sperm extraction (TESE). Therefore, we point out the importance of genetic testing and counselling regarding Y chromosome microdeletion for couples requesting ICSI.