Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line
Articles > Article · 2016
Authors: Derya İLEM ÖZDEMİR, Sinem Yaprak KARAVANA, ZEYNEP ŞENYİĞİT, ÇAĞRI ÇALIŞKAN, Meliha EKİNCİ, MAKBULE AŞIKOĞLU, ESRA BALOĞLU
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY, Journal Indexed in SCI Expanded, International, 2016, vol 310, pp 515-522
DOI: 10.1007/s10967-016-4805-6
Summary
In the present study incorporation differences between the developed formulations for treatment for bladder cancer was investigated by radioactive cell culture studies. Developed bioadhesive microspheres (MS) and gemcitabinehydrochloride loaded MS (GHCl-MS) were radiolabeled. After observing the optimum labeling conditions, Tc-99m-MS and Tc-99m-GHCl-MS were loaded to the chitosan gel (CG) and poloxamer gel. The in vitro cell incorporation affinity of newly developed formulations to bladder papilloma (RT4) and the carcinoma (T24) cell lines was investigated. Cell culture studies results indicate that the use of prolonged release bioadhesive CG formulation was highly improved the targeting of the GHCl to the cancer cells.