Arginine selective biosensor based on arginase-urease immobilized in gelatin
Articles > Article · 2003
Authors: SELMA KARACAOĞLU, Suna TİMUR, AZMİ TELEFONCU
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, Journal Indexed in SCI Expanded, International, 2003, vol 31, pp 357-363
Summary
Arginase and urease enzymes were immobilized on the surface of pH electrode by using gelatin membrane which is then cross-linked with glutaraldehyde. Sensor response was maximum when 2.5 mM, Tris-HCl buffer (pH 8.5) was used at 25degreesC. The biosensor response depends linearly on arginine concentration between 0.025-0.310 mM with response time 10 min. Furthermore, application of the system for the arginine detection in serum samples was also tested.