Evaluation Of VEGF, EGF-R AND TGF-alpha In Placenta Of Pregnancies With Preeclampsia By Immunohistochemistry
Articles > Article · 2008
Authors: Teksin Cirpanli, Fuat Akercan, Mustafa Cosan Terek, Hasan Tayfun Ozcakir, Gulsen Giray, Sermet SAĞOL, Nedim Karadadas
TURKISH JOURNAL OF OBSTETRICS AND GYNECOLOGY, Journal Indexed in SCI Expanded, International, WoS, 2008, vol 5, pp 40-45
Summary
Objective: The aim of the study is to determine VEGF, EGFR and TGF-alpha with the immunohistochemical staining in placenta biopsies of patients with preeclampsia.Design: Prospective case control study. Materials and Methods: Biopsies from the placenta were obtained from ten patients with preeclapmsia and ten patients of control group during the cesarean section. Placenta biopsies were examined for VEGF, EGF-R and TGF-alpha distribution with avidin-biotinperoxidase immunohistochemistry. The staining intensities were graded semi-quantitatively by the two histologists who were blinded to the groups and the H-score was calculated by light microscopic examination.Results: The VEGF, EGFR and TGF-alpha expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2 +/- 22.65 versus 201.9 +/- 12.33, p=0.000; 186.3 +/- 4.98 versus 150.3 +/- 5.7, p=0.000; 185.1 +/- 7.48 versus 169.2 +/- 6.19, p=0.000; respectively).Conclusion: Immunostaining of VEGF, EGF-R and TGF-alpha is significantly higher in placenta biopsies in patients with preeclampsia.