P0334 TLR4 GENE MUTATION IN HOST DEFENCE AGAINST HEPATITIS B VIRUS
Bildiriler > Özet Bildiri
Yazarlar: Funda ÇETİN, A. Berdeli, C. Arikan, S. Aydogdu, R. V. Yagci
Journal of Pediatric Gastroenterology and Nutrition, , , Uluslararası, Özet Bildiri, Wos, cilt 39, ss S184-S184
DOI: 10.1097/00005176-200406001-00458
Özet
Introduction: Pathogen recognition and activation of innate and adaptive immune response is mediated by the members of human Toll-like receptor (TLR) family. We aimed to evaluate TLR4 gene polymorphism in constitution of various immune responses against hepatitis B virus (HBV). Methods: Seventy-six subjects, asymptomatic hepatitis B surface antigen (HbsAg) carriers (group I, n:15), naturally immune cases (group II, n:16) and chronic hepatitis B infected children (groupIII, n:35) were inrolled in the study. Chronic hepatitis B infection was determined by presence of HbsAg, HbeAg and HBV DNA in serum screened at 3 months intervals for at least 1 year, serum alanine transaminase levels more than 1,5 times the upper normal limit and chronic hepatitis with histologic activity index (HAI) more than 5 by liver biopsy. TLR4 gene mutations were studied by the PCR-RFLP method. Incidence of polymorphism was compared between the three groups. Results: Homozygous TLR4 gene Thr399Ile mutation was detected in one patient (6,7%) in-group I. Among chronic hepatitis B infected children (group III), 7 patients (20%, 1 homozygous, 6 heterozygous) had TLR4 gene mutation. Two patients in-group III was siblings with TLR4 heterozygote mutation and one of the parents was also infected chronically with HBV. No mutation was determined in the naturally immune cases. The incidence of TLR4 gene polimorphism was not different between the groups (p:0.09). Conclusion: Our preliminary data adresses a higher incidence of TLR4 gene mutation in chronic hepatitis B infected children, although statistically non-significant. TLR4 gene might determine the constitution of host response against hepatitis B virus.