Determination of Xanthine Oxidase by Microcalorimetric Method
Makaleler > Tam Makale · 1982
Yazarlar: S. Yildiz, B. Pekin
Analytical Letters, Uluslararası, 1982, cilt 15, ss 405-412
DOI: 10.1080/00032718208064392
Özet
In this study, the enzymatic oxidation of acetaldehyde, one of the substrates of xanthine oxidase, has been studeid by a microcalorimetric method. A flow microcalorimeter is used to measure the heat changes during the during the reaction. The catalytic determination of xanthine oxidase between the concentrations 0.2×10−1 and 2.4×10−1 mg/ml (0.8×10−3 −9.6×10−3 U/ml) has been achived keeping acetaldehyde concentration constant at 4.0×10−3 M and the catalymetric determination of acetaldehyde between 0.8×10−3 and 2.8×10−3 M is accomplished at a fixed concentration of enzyme.