Quantitative determination of lycorine in Amaryllidaceae plants
Makaleler > Tam Makale · 2009
Yazarlar: GI Kaya, D Cicek, B Sarıkaya, MA Onur, Nehir SOMER
Planta Medica, Uluslararası, Wos, 2009, cilt 75
Özet
Lycorine, a widespread alkaloid in Amaryllidaceae species, has been proven to have antiviral, cytotoxic and antimalarial activities [1–3]. A reversed-phase high-performance liquid chromatographic method has been developed and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut ® ) [4]. The chromatographic separation was performed using an isocratic system with a mobile phase trifluoroacetic acid-water-acetonitrile (0.01:90:10) and diode array detector [5]. The linearity of the method was studied by injecting five known concentrations of the standard in the range of 62.5–500µgmL -1 . The calibration curve was determined as Y=23.9788285 X + 62.391901. Validation procedures showed that the method was specific, accurate and precise. The above-mentioned method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. collected during two different vegetation periods. Considering the botanical parts (aerial parts, bulbs) and vegetation periods (flowering and fruiting), there were significant differences in the content of lycorine. The amount of lycorine in S. sicula ranged between 0.097–0.529%, and the highest amount was found in the aerial parts collected during flowering period. The lycorine content in S. lutea and in Pancratium maritimum ranged between 0.195–0.399% and 0.048–0.145%, respectively. The highest content of lycorine in these two species was detected in the bulbs collected during the fruiting period. References: [1] Gabrielsen, B. et al. (1992)J. Nat. Prod. 55:1569–1581. [2] Liu, J. et al. (2004), FEBS Lett. 578:245–250. [3]Şener, B. et al. (2003) Phytother. Res. 17:1220–1223. [4] Berkov, S. et al. (2008) Phytochem. Anal. 19:285–293. [5] Mustafa, N.R. et al. (2003)J. Liq. Chromatogr. R. T. 26:3217–3233.