Development of Fluorescent Array Based on Sol-Gel/Chitosan Encapsulated Acetylcholinesterase and pH Sensitive Oxazol-5-one Derivative
Makaleler > Tam Makale · 2010
Yazarlar: Gulsiye ÖZTÜRK, Karl-Heinz FELLER, Kay DORNBUSCH, Suna TİMUR, Serap Alp, Yavuz Ergun
JOURNAL OF FLUORESCENCE, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2010, cilt 21, ss 161-167
DOI: 10.1007/s10895-010-0700-7
Özet
A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane / chitosan matrix via a microarrayer. The constructed tetraethoxysilane / chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 x 10(-8) M, with a detection limit of 0.27 x 10(-8) M.