An amperometric inhibitor biosensor for the determination of reduced glutathione (GSH) without any derivatization in some plants
Makaleler > Tam Makale · 2004
Yazarlar: MK SEZGINTURK, Erhan DİNÇKAYA
BIOSENSORS & BIOELECTRONICS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2004, cilt 19, ss 835-841
DOI: 10.1016/j.bios.2003.08.012
Özet
In this study, an amperometric biosensor based on cucumber tissue homogenate was developed for the determination of glutathione. Cucumber (Cucumis sativus L.) tissue homogenate was used as the biological material. The cucumber tissue homogenate was cross-linked with gelatine using glutaraldehyde and fixed on a pretreated teflon membrane. The principle of the measurements was based on the determination of the decrease in the differentiation of oxygen level which had been caused by the inhibition of ascorbate oxidase in the biological material by glutathione. Determinations were carried out by standard curves which were obtained by the measurement of the decrease in the consumed oxygen level related to glutathione concentration.