The effect of pretreatment with substrates on the activity of immobilized pancreatic lipase
Articles > Article · 2013
Authors: Güliz AK DEMİROZ, Yeser Aktuna, Funda KARTAL, Ali KILINÇ
Artificial Cells, Nanomedicine, and Biotechnology, Journal Indexed in SCI Expanded, International, Scopus, 2013, vol 42, pp 146-150
DOI: 10.3109/21691401.2013.764312
Summary
Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker. The effect of pre-treatment of lipase previously with various types of oils on immobilization efficiency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.