A novel 2 bp deletion variant in Ovine-DRB1 gene is associated with increased Visna/maedi susceptibility in Turkish sheep
Diğer yayınlar > Diğer · 2021
Yazarlar: Yalçın Yaman, Veysel BAY, Ramazan Aymaz, Murat Keleş, Yasemin Öner, Eden Yitna Teferedegn, Cemal ÜN
Diğer, Uluslararası, 2021,
Özet
Abstract Visna/Maedi (VM) is a multisystemic lentivirus infection of sheep that affecting sheep industry across the globe. TMEM154 gene has been identified to be a major VM associated host gene, nevertheless, a recent study showed that the frequency of the VM-resistant TMEM154 haplotypes was very low or absent in indigenous sheep. Thus, the present study was designed to determine other possible co-receptors associated with VM. For this purpose, DRB1 gene, which is renowned for its role in host immune response activity against various diseases was targeted. A total number of 151 case-control matched pairs were constructed from 2266 ELISA tested sheep. A broad range of DRB1 haplotype diversity was detected by sequence-based genotyping. Moreover, a novel 2 bp deletion (del) in the DRB1 intron 1 was identified. For final statistic, the sheep carrying VM-resistant TMEM154 diplotypes were removed and a McNemar’s test with a matched pairs experimental design was conducted. Consequently, it was identified for the first time that the 2 bp del variant is a genetic risk factor for VM (p-value, 0.002; chi-square, 9.3; odds ratio, 2.9; statistical power > 0.93) in dominant model. Thus, negative selection for 2 bp del variant could decrease VM infection risk in Turkish sheep.