Urate oxidase electrode based on dissolved oxygen probe for urine uric acid determination
Makaleler > Tam Makale · 2000
Yazarlar: Erhan DİNÇKAYA, Erol AKYILMAZ, Sinan AKGÖL
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2000, cilt 37, ss 67-70
Özet
A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35 degrees C. The enzyme electrode response depends linearly on uric acid concentration between 5-40 mu M with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed.